Sensitive determination of free and plasma protein-bound dipyridamole by high-performance liquid chromatography.
Fiche publication
Date publication
avril 1991
Journal
Journal of chromatography
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BARBERI-HEYOB Muriel, Pr MERLIN Jean-Louis
Tous les auteurs :
Barberi M, Merlin JL, Weber B
Lien Pubmed
Résumé
For many years dipyridamole (DP) has been used in the treatment of hypertension as a vasodilator, but recently it has been recognised as an anti-platelet aggregation agent and to potentiate anti-metabolite activity. A rapid and sensitive (20 nM) procedure for the determination of free and protein-bound DP in plasma, using reversed-phase high-performance liquid chromatography on an Ultrasphere XL ODS (3 microns) column (70 mm x 4.6 mm I.D.) with ultraviolet detection (280 nm), is reported. Free and bound DP were separated using ultrafiltration. Concentrations of DP between 0.1 and 10 microM were measured in plasma with a relative standard deviation of less than 9.6%. The subsequent determination of DP levels in patients orally administered 450 mg per day showed that DP binding to plasma protein is higher than 90%.
Mots clés
Administration, Oral, Chromatography, High Pressure Liquid, methods, Dipyridamole, administration & dosage, Female, Humans, Male
Référence
J. Chromatogr.. 1991 Apr;565(1-2):511-5