MTT assays allow quick and reliable measurement of the response of human tumour cells to photodynamic therapy.
Fiche publication
Date publication
janvier 1992
Journal
European journal of cancer (Oxford, England : 1990)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MERLIN Jean-Louis
Tous les auteurs :
Merlin JL, Azzi S, Lignon D, Ramacci C, Zeghari N, Guillemin F
Lien Pubmed
Résumé
MCF-7 and HT-29 cell lines were selected as a reliable model to examine the possible parameters affecting the sensitivity of tumour cells to photodynamic therapy (PDT) using a dye-laser at 630 nm. The chemical composition of haematoporphyrin derivative (HPD) was determined by high-performance liquid chromatography (HPLC) analysis and was in agreement with reported values. MTT assays were performed to assess the time-dependency of PDT and the influence of the output power and light fluence. The results showed a maximal cytotoxicity 48 h after photoirradiation. The output power (1 or 2 W) did not significantly affect the cytotoxicity when the fluence was constant (20 J/cm2). However, an increase in fluence (10-40 J/cm2) led to a significant enhancement of cytotoxicity until maximal values were reached (30-40 J/cm2). A further increase in fluence (50 J/cm2) proved to induce a fall-off in cytotoxicity related to the intense photobleaching of HPD.
Mots clés
Adenocarcinoma, drug therapy, Breast Neoplasms, drug therapy, Chromatography, High Pressure Liquid, Colonic Neoplasms, drug therapy, Female, Hematoporphyrin Derivative, Hematoporphyrins, chemistry, Humans, Photochemotherapy, methods, Radiation-Sensitizing Agents, chemistry, Sensitivity and Specificity, Tetrazolium Salts, Thiazoles, Tumor Cells, Cultured, drug effects
Référence
Eur. J. Cancer. 1992 ;28A(8-9):1452-8