Analysis of 5-fluorouracil in plasma and urine by high-performance liquid chromatography.
Fiche publication
Date publication
octobre 1992
Journal
Journal of chromatography
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BARBERI-HEYOB Muriel, Pr MERLIN Jean-Louis
Tous les auteurs :
Barberi-Heyob M, Merlin JL, Weber B
Lien Pubmed
Résumé
A relatively simple, sensitive and rapid high-performance liquid chromatographic method is described for measuring the anticancer drug 5-fluorouracil (5-FU) in human plasma and urine. The procedure includes liquid-liquid extraction using ethyl acetate-methanol (95:5) and preparative column chromatography to separate 5-FU from constituents normally occurring in these biological samples. The columns contained a specially modified form of diatomaceous earth, which requires no pre-conditioning washes. Reversed-phase high-performance liquid chromatography was performed on a C18 column (70 mm x 4.6 mm I.D.) with a mobile phase of water-methanol (95:5) and ultraviolet detection (268 nm). The overall recovery from plasma and urine was 91 and 94%, respectively, at the concentration of 50 ng/ml. The determination limit of the assay for 5-FU was 10 ng/ml of plasma and urine. Concentrations of 5-FU between 10 and 500 ng/ml were measured in plasma and urine with a relative standard deviation of 6.8%. In order to evaluate the procedure, plasma and urine samples from three patients treated with 5-FU by continuous intravenous perfusion, were investigated.
Mots clés
Breast Neoplasms, blood, Chromatography, High Pressure Liquid, methods, Drug Monitoring, methods, Female, Fluorouracil, analysis, Humans, Reproducibility of Results, Spectrophotometry, Ultraviolet
Référence
J. Chromatogr.. 1992 Oct;581(2):281-6