Evaluation of Taxol in head and neck squamous carcinoma multicellular tumor spheroids.
Fiche publication
Date publication
janvier 1997
Journal
Anti-cancer drugs
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MERLIN Jean-Louis
Tous les auteurs :
Griffon-Etienne G, Merlin JL, Marchal C
Lien Pubmed
Résumé
Taxol cytotoxicity was evaluated in human head and neck squamous carcinoma cell lines growing as multicellular tumor spheroids (MTS) and compared with monolayered culture using conventional clonogenic assays. End points were respectively the concentration inhibiting 50% of the cellular growth (IC50) in clonogenic assays and the concentration required to induce a 50% decrease in the MTS volume (ID50) or number in the overall spheroid population (SCC50). A significant difference was observed when the cells were exposed for 10 days to Taxol as a consequence of the different growth kinetics of the spheroids. After 16 day exposure of spheroids to Taxol, no difference remained between ID50 and IC50. In addition, a significant correlation was found between individual spheroid sensitivity to Taxol (ID50) and the spheroid population sensitivity (SCC50). Both parameters (ID50 and SCC50) defined in cell models appear useful for the evaluation of chemosensitivity of three-dimensional structures known to be closer to in vivo tumor models.
Mots clés
Antineoplastic Agents, Phytogenic, therapeutic use, Carcinoma, Squamous Cell, drug therapy, Cell Division, drug effects, Cell Line, Head and Neck Neoplasms, drug therapy, Humans, Paclitaxel, therapeutic use, Spheroids, Cellular, cytology, Tumor Stem Cell Assay
Référence
Anticancer Drugs. 1997 Jan;8(1):48-55