Combined flow cytometry and immunohistochemistry analyses for the assessment of nasopharyngeal carcinoma cell kinetics by in vivo bromodeoxyuridine infusion.
Fiche publication
Date publication
octobre 1997
Journal
Cytometry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MERLIN Jean-Louis
Tous les auteurs :
Marchal S, Marchal C, Parache RM, Benjaafar N, Odda M, Leclerc A, Merlin JL, el Gueddarri B, Bey P
Lien Pubmed
Résumé
Thirty-eight previously untreated patients with a histologically proved diagnosis of nasopharyngeal carcinoma were evaluated for in vivo cell kinetics before treatment by conventional radiation therapy. Thirty-seven tumors were analyzed by flow cytometry. Values of median potential doubling time (Tpot), labelling index (LI), and duration of S phase (Ts) were, respectively, 10.9 days, 3.8%, and 10.8 hours. In 35 cases, the results obtained from two biopsies of the same tumor were compared. A good reproducibility was obtained for LI and Tpot (P < 0.01). Thirty-one tumors were analysed by immunohistochemistry and labelling index (HLI) was determined in 24 tumors with a percentage of labelled cells varying from 6.1% to 39.2% (median value = 18.5%). No correlation was found between LI and HLI, but when observations were focused on the restricted group of DNA aneuploid samples, mean values of LI and HLI were closer (respectively, 12.8 +/- 4.5% and 18.3 +/- 7.7%) and a good correlation was obtained (P = 0.01). Moreover, no difference in proliferation was found between diploid and aneuploid tumors. Considering these results, a combined Tpot was calculated that allowed classification of tumors as highly or slowly proliferative.
Mots clés
Adult, Aged, Bromodeoxyuridine, administration & dosage, Carcinoma, metabolism, Flow Cytometry, methods, Humans, Immunohistochemistry, methods, Infusions, Intravenous, Kinetics, Middle Aged, Nasopharyngeal Neoplasms, metabolism, Reproducibility of Results
Référence
Cytometry. 1997 Oct;29(2):165-72