Specific fluorescent tracers. Imaging and applications for photodynamic therapy.
Fiche publication
Date publication
avril 2002
Journal
Comptes rendus biologies
Auteurs
Membres identifiés du Cancéropôle Est :
Dr FROCHOT Céline, Pr MERLIN Jean-Louis
Tous les auteurs :
Teiten MH, Even P, Burgos P, Frochot C, Aubert S, Carré MC, Bolotine L, Merlin JL, Guillemin F, Viriot ML
Lien Pubmed
Résumé
Our main objective is to enlarge the fluorescence use in biosciences, with especially the photodynamic therapy (PDT) used for cancer treatment as one of the target applications. Meta-tetra(hydroxyphenyl)chlorin (m-THPC) is a second-generation photosensitiser, applied in photodynamic therapy. The localisation of this sensitiser as well as its induced cell death mechanisms in human breast cancer cells (MCF-7 and its resistant subline MCF-7DXR, DXR: doxorubicin) were evaluated using fluorescence microscopy. In addition, we will present two additional routes, whose aims are to create new features to respond to the PDT questioning: firstly, the synthesis of fluorescent tracers, with a particular attention to the presence of hydrophilic groups (glucosamine ring) on the basic fluorophore structure to orientate the localisation of the probe and, secondly, the use of scanning near-field optical microscopy to reach a better resolution for the fluorescence microscopy analysis.
Mots clés
Biological Transport, Breast Neoplasms, pathology, Cell Survival, drug effects, DNA, Neoplasm, metabolism, Drug Resistance, Neoplasm, Female, Fluorescent Dyes, Glycosylation, Humans, Lysosomes, metabolism, Mesoporphyrins, pharmacokinetics, Microscopy, Fluorescence, methods, Neoplasms, drug therapy, Photochemotherapy, methods, Porphyrins, chemistry, Radiation-Sensitizing Agents, pharmacokinetics, Subcellular Fractions, metabolism, Tumor Cells, Cultured
Référence
C. R. Biol.. 2002 Apr;325(4):487-93