Enhanced gene transfer and cell death following p53 gene transfer using photochemical internalisation of glucosylated PEI-DNA complexes.
Fiche publication
Date publication
août 2004
Journal
The journal of gene medicine
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DOLIVET Gilles, Dr LEROUX Agnès, Pr MERLIN Jean-Louis
Tous les auteurs :
Ndoye A, Merlin JL, Leroux A, Dolivet G, Erbacher P, Behr JP, Berg K, Guillemin F
Lien Pubmed
Résumé
p53 is frequently mutated in many cancers including human head and neck squamous cell carcinoma and pancreatic cancer. In tumor models, wild-type (wt) p53 gene transfer induces apoptosis and tumor regression in vivo, justifying the extensive clinical investigation of p53 gene therapy.
Mots clés
Apoptosis, DNA Fragmentation, Gene Transfer Techniques, Green Fluorescent Proteins, genetics, Humans, Immunohistochemistry, Indicators and Reagents, chemistry, Microscopy, Fluorescence, Photochemistry, Plasmids, Polyethyleneimine, analogs & derivatives, RNA, Messenger, metabolism, Tumor Cells, Cultured, Tumor Stem Cell Assay, methods, Tumor Suppressor Protein p53, genetics
Référence
J Gene Med. 2004 Aug;6(8):884-94