An In Vitro Model for Fibroblast Photoaging Comparing Single and Repeated UVA Irradiations.
Fiche publication
Date publication
juin 2017
Journal
Photochemistry and photobiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HUMBERT Philippe
Tous les auteurs :
Nakyai W, Saraphanchotiwitthaya A, Viennet C, Humbert P, Viyoch J
Lien Pubmed
Résumé
The current method for efficient evaluation of anti-photoaging compounds is an in vitro skin culture model using a single ultraviolet A (UVA) irradiation of fibroblasts. However, skin photoaging is caused by repeated exposure to UVA radiation. The objective of this study was to develop an appropriate model for in vitro skin photoaging by comparing the different effects of single (5 J/cm(2) ) and repeated exposures (5 J/cm(2) x 3 times) of fibroblasts to UVA irradiation. Our results demonstrated that a single and repeated exposure to UVA irradiation had different effects on fibroblasts. In the single UVA-irradiated group, collagen lattice contraction and the protein levels of type I procollagen and matrix metalloproteinase-1 (MMP-1) increased, while the levels of fibronectin and alpha-smooth muscle actin (α-SMA) were unchanged, compared to levels in the non-UVA irradiated group (control). In contrast, repeated UVA exposure significantly induced G0/G1 cell cycle arrest, reduced collagen lattice contraction and type I procollagen and fibronectin expression, and increased MMP-1 expression. There was no difference in α-SMA expression when comparing repeatedly irradiated and non-UVA irradiated fibroblasts. Our findings clearly indicate that repeated UVA irradiation of cells induces malfunctions found in photoaged skin and is an appropriate in vitro skin model of photoaging. This article is protected by copyright. All rights reserved.
Référence
Photochem. Photobiol.. 2017 Jun;: