Structural and functional evidence for ligand-independent transcriptional activation by the estrogen-related receptor 3.
Fiche publication
Date publication
février 2002
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah, Dr MORAS Dino, Dr VAN DORSSELAER Alain
Tous les auteurs :
Greschik H, Wurtz JM, Sanglier S, Bourguet W, van Dorsselaer A, Moras D, Renaud JP
Lien Pubmed
Résumé
The crystal structure of the ligand binding domain (LBD) of the estrogen-related receptor 3 (ERR3) complexed with a steroid receptor coactivator-1 (SRC-1) peptide reveals a transcriptionally active conformation in absence of any ligand. The structure explains why estradiol does not bind ERRs with significant affinity. Docking of the previously reported ERR antagonists, diethylstilbestrol and 4-hydroxytamoxifen, requires structural rearrangements enlarging the ligand binding pocket that can only be accommodated with an antagonist LBD conformation. Mutant receptors in which the ligand binding cavity is filled up by bulkier side chains still interact with SRC-1 in vitro and are transcriptionally active in vivo, but are no longer efficiently inactivated by diethylstilbestrol or 4-hydroxytamoxifen. These results provide structural and functional evidence for ligand-independent transcriptional activation by ERR3.
Référence
Mol Cell. 2002 Feb;9(2):303-13.