Detection of the human papillomavirus and analysis of the TP53 polymorphism of exon 4 at codon 72 in penile squamous cell carcinomas.
Fiche publication
Date publication
mars 2003
Auteurs
Membres identifiés du Cancéropôle Est :
Pr AUBIN François, Pr BITTARD Hugues
Tous les auteurs :
Humbey O, Cairey-Remonnay S, Guerrini JS, Algros MP, Mougin C, Bittard H, Aubin F
Lien Pubmed
Résumé
Human papillomaviruses (HPV) are thought to be involved in penile squamous cell carcinomas (SCC). A common polymorphism at codon 72 of exon 4 encoding either arginine (Arg) or proline (Pro) has been shown to affect HPV-mediated degradation of p53 in vitro, and may represent a risk factor for HPV-induced carcinogenesis. The presence of HPV DNA as well as the TP53 polymorphism at codon 72 of exon 4 were investigated in a series of 45 penile SCC. HPV detection and typing were carried out by polymerase chain reaction (PCR) with generic primers (MY09-MY11 and FAP59-FAP64), and type-specific DNA probes. TP53 polymorphism was further investigated using Denaturing Gradient Gel Electrophoresis (DGGE). HPV DNA was detected in 67% of penile SCC and 32% of benign lesions (BL) (P
Référence
Eur J Cancer. 2003 Mar;39(5):684-90.