Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes.
Fiche publication
Date publication
juin 2005
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves
Tous les auteurs :
Shynkar VV, Klymchenko AS, Duportail G, Demchenko AP, Mely Y
Lien Pubmed
Résumé
The dipole potential (Psi(d)) constitutes a large and functionally important part of the electrostatic potential of cell plasma membranes. However, its direct measurement is not possible. Herein, new 3-hydroxyflavone fluorescent probes were developed that respond strongly to Psi(d) changes by a variation of the intensity ratio of their two well-separated fluorescence bands. Using fluorescence spectroscopy with cell suspensions and confocal microscopy with adherent cells, we showed, for the first time, two-color fluorescence ratiometric measurement and visualization of Psi(d) in cell plasma membranes. Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced. (c) 2005 Elsevier B.V. All rights reserved.
Référence
Biochim Biophys Acta. 2005 Jun 30;1712(2):128-36.