Characterisation of uterine sarcoma cell lines exhibiting MDR phenotype by vibrational spectroscopy.
Fiche publication
Date publication
novembre 2005
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SOCKALINGUM Ganesh
Tous les auteurs :
Murali Krishna C, Kegelaer G, Adt I, Rubin S, Kartha VB, Manfait M, Sockalingum GD
Lien Pubmed
Résumé
Multidrug resistance (MDR) enables cancer cells to escape cytotoxic insults of anticancer drugs. Rapid identification of cells exhibiting the MDR phenotype is very important since it can lead to an effective and individual patient based treatment plan. We have investigated a combined vibrational spectroscopic approach, using both micro-Raman and FTIR techniques, in order to characterise a sensitive human uterine sarcoma cell line MES-SA and its multidrug-resistant derivative Garf. In this study, these two complementary methods have been evaluated via the use of principal components analysis (PCA), for discrimination of cells exhibiting the MDR phenotype. Our results indicate that, though they inherently have different sensitivities, both Raman and IR methods can provide a good differentiation of cell phenotypes.
Référence
Biochim Biophys Acta. 2005 Nov 15;1726(2):160-7