Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin.
Fiche publication
Date publication
février 2006
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MORAS Dino, Dr RUFF Marc
Tous les auteurs :
Syakhovich VE, Saraswathi NT, Ruff M, Bokut SB, Moras D
Lien Pubmed
Résumé
Human glycosylated haemoglobin A(1C) is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA(1C) were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 angstrom at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 angstrom, alpha = 90.00, beta = 125.40, gamma = 90.00 degrees. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight (V-M) of 9.70 angstrom(3) Da(-1) and a solvent content of 49%.
Référence
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt