Sensing of adenosine-5'-triphosphate anion in aqueous solutions and mitochondria by a fluorescent 3-hydroxyflavone dye.
Fiche publication
Date publication
octobre 2007
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves
Tous les auteurs :
Yushchenko DA, Vadzyuk OB, Kosterin SO, Duportail G, Mely Y, Pivovarenko VG
Lien Pubmed
Résumé
The current work demonstrates the formation of complexes between the tetraanion acienosine-5'-triphosphate (ATP) and the flavone derivative 3-hydroxy-4'-(dimethylamino)flavone (FME). Two kinds of complexes are evidenced. The higher affinity ATP-FME complex corresponds to a stacking of the two aromatic molecules and leads to a strong hypochromicity of the absorption spectrum of the dye. The lower affinity (ATP)(2)-FME complex results in a strong increase of the fluorescence intensity (similar to 20-fold), due mainly to the appearance of the anionic form of FME, as shown by the important red shift (60 nm) of both excitation and emission spectra. Molecular modeling indicates that this anionic form results from the deprotonation induced by the influence of the tetra-charged triphosphate group of the ATP molecules. Using its strong enhancement of fluorescence intensity in the presence of ATP, the dye was used successfully to monitor the succinate-induced production of endogenous ATP in mitochondria. As a consequence, FME can be considered as a starting point to design efficient ATP sensors. (c) 2007 Elsevier Inc. All rights reserved.
Référence
Anal Biochem. 2007 Oct 15;369(2):218-25