Requirements for PCHA monoubiquitination in human cell-free extracts
Fiche publication
Date publication
décembre 2007
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CORDONNIER Agnès
Tous les auteurs :
Schmutz V, Wagner J, Janel-Bintz R, Fuchs RPP, Cordonnier AM
Lien Pubmed
Résumé
The Rad6/Rad18-dependent monoubiquitination of PCNA plays a crucial role in regulating replication past DNA damage in eukaryotic cells. We show here that in human cell-free extracts, efficient PCNA monoubiquitination requires both the synthesis of relatively long DNA tracts and polymerase idling or stalling at sites of DNA modification or DNA secondary structures. This dual dependency suggests a dynamic process in which, following initiation, the DNA synthesizing complex undergoes modifications that make it competent as a mediator for the activation of the Rad6/Rad18 pathway.
Référence
DNA Repair (Amst). 2007 Dec 1;6(12):1726-31