A direct urinary free cortisol RIA compared with GC-MS on quality control material
Fiche publication
Date publication
décembre 2007
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AGIN Arnaud
Tous les auteurs :
Sapin R, Agin A, Gasser F
Lien Pubmed
Résumé
Aim of the study. - To evaluate the specificity of a direct urinary free cortisol assay. Material and methods. - We studied 27 urinary samples from the Probioqual external quality assessment program. We compared the results obtained in our laboratory using a direct RIA (Immunotech IM1841) with those determined by Probioqual using the reference method, gas chromatography-mass spectrometry (GC-MS). Results. - Correlation between the results of the two methods was good (r = 0.978) and the regression line was the following: RIA (nmol/L) = 1.10 GC-MS (nmol/L) - 3.4. All relative differences but one lay between -20 and +30% and no clear discordance was observed. Conclusion. - This study on quality control material confirms the good specificity of the direct RIA previously observed on clinical samples from untreated and corticoid treated patients. (C) 2007 Elsevier Masson SAS. Tous droits reserves.
Référence
Immuno-anal Biol Spec. 2007 Dec;22(6):398-400