Identification of two populations of osteoarthritic osteoblasts according to the 1,25[OH](2) vitamin D(3) potency to stimulate osteocalcin.
Fiche publication
Date publication
janvier 2015
Auteurs
Membres identifiés du Cancéropôle Est :
Pr JOUZEAU Jean-Yves, Pr MAINARD Didier
Tous les auteurs :
Hu Y, Liu Y, Lajeunesse D, Mainard D, Jouzeau JY, Reboul P
Lien Pubmed
Résumé
INTRODUCTION: Few studies have tried to discriminate a differential behavior between osteoarthritic (OA) osteoblasts (Obs). Based on osteocalcin level, we aimed, in the present study, to evaluate the capacity of OA Obs for producing molecules of the Wnt/beta-catenin signaling pathway. METHODS: Human primary OA Obs (n=11) were exposed or not to 50 nM of 1,25 dihydroxyvitamin D3 (VitD3) for 24 h. Osteocalcin (OCN), TGF-beta1, Dickkopf-related protein 2 (DKK2), R-spondin 2 (Rspo2), Wnt5b, and low density lipoprotein related-receptor 1 (LRP1) were evaluated by real time RT-PCR. RESULTS: All samples responded to VitD3 as validated by the increase in OCN expression. However two populations of Obs were discriminated; one called "high responders" whose OCN stimulation was higher than 100 fold (mean 881 fold, p
Référence
Biomed Mater Eng. 2015;25(1 Suppl):103-10