Cell guidance into quiescent state through chromatin remodeling induced by elastic modulus of substrate.
Fiche publication
Date publication
janvier 2015
Auteurs
Membres identifiés du Cancéropôle Est :
Dr FREUND Jean-Noël, Dr LAVALLE Philippe, Dr VOEGEL Jean-Claude, Pr SCHAAF Pierre, Dr RABINEAU Morgane
Tous les auteurs :
Rabineau M, Flick F, Mathieu E, Tu A, Senger B, Voegel JC, Lavalle P, Schaaf P, Freund JN, Haikel Y, Vautier D
Lien Pubmed
Résumé
Substrate stiffness is known to strongly influence the fate of adhering cells. Yet, little is known about the influence of the substrate stiffness on chromatin. Chromatin integrates a multitude of biochemical signals interpreted by activation or gene silencing. Here we investigate for the first time the organization of chromatin of epithelial cells on substrate with various mechanical properties. On stiff substrates (100-200 kPa), where cells preferentially adhere, chromatin is mainly found in its euchromatin form. Decreasing the Young modulus to 50 kPa is correlated with a partial shift from euchromatin to heterochromatin. On very soft substrates (
Référence
Biomaterials. 2015 Jan;37:144-55