Elecsys (R) prolactin immunoassays: from Prolactin to Prolactin II
Fiche publication
Date publication
avril 2008
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AGIN Arnaud
Tous les auteurs :
Sapin R, Le Guyader E, Agin A, Gasser F
Lien Pubmed
Résumé
Aim of the study. - To evaluate the reactivity of the new Elecsys (R) protactin II (PRLII) immunoassay with macroprolactin. Material and methods. - We compared the results of the two Elecsys (R) prolactin assays from Roche Diagnostics, the old protactin (PRL) and the new protactin II in 213 hyperprotactinernic sera as measured with the PRL assay. Macroprolactin was determined after polyethylene glycol (PEG) precipitation using the PRL assay (MPRL). One hundred and two sera were considered as macroprolactin negative (MPRL < 50%), 87 positive (MPRL >= 60%) and 24 undetermined. Monomeric prolactin was measured with the PRL assay (PRLm) in all samples and with the PRLII assay (PRLIIm) in 142 from the 144 samples with increased PRLII values. Results. - In the negative group, PRL and PRLII results were perfectly correlated (r = 0.998) but PRLII values were 20-30% lower than PRL values. In the positive group, macroprolactin was detected to a lesser extent by the PRLII assay than by the PRL assay (49 sera with increased PRL results had PRLII results within the reference interval) but the PRLII assay was still reactive with macroprolactin (among 82 sera with PRLm within the reference interval 33 had increased PRLII results). Correlation (r = 0.98 and concordance (95%) between PRLm and PRLIIm values determined in 142 sera were good. Conclusion. - The prolactin II assay reacts less strongly than the prolactin assay with macroprolactin. However, the PEG precipitation and monomeric protactin determination are still required to confirm hyperprolactinemia. (C) 2008 Elsevier Masson SAS. Tous droits reserves.
Référence
Immuno-anal Biol Spec. 2008 Apr;23(2):103-8