Evaluation of early nerve regeneration using a polymeric membrane functionalized with nerve growth factor (NGF) after a crush lesion of the rat mental nerve.
Fiche publication
Date publication
avril 2008
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LAVALLE Philippe
Tous les auteurs :
Savignat M, Vodouhe C, Ackermann A, Haikel Y, Lavalle P, Libersa P
Lien Pubmed
Résumé
Purpose: The aim of this study was to evaluate mental nerve regeneration in rats after a crush lesion by using a polymeric membrane functionalized with nerve growth factor (NGF). Materials and Methods: To evaluate the regeneration 1 month after the lesion, electrophysiological recordings of afferent neurograms were associated with a histologic analysis of neurons soma in the trigeminal ganglion using a retrograde fluorescent tracer. Three animal groups were used: uninjured rats (TEM), injured control rats without membrane (CONT), and injured rats with membrane containing NGF. Results: One month after the crush injury, an early regeneration was observed independently of exogenous NGF. However, in comparison with the level recorded before the lesion, the afferent activity was decreased by 28.5% in the CONT group, whereas it was increased by 30.8%, in the NGF group. In comparison with the TEM group, a decrease in the mean number of labeled neurons was observed in the CONT group, whereas no significant difference was found after 1 month of NGF treatment. Conclusion: The exogenous application of NGF using a polymeric membrane improves mental nerve regeneration after a crush lesion. (C) 2008 American Association of Oral and Maxillofacial Surgeons.
Référence
J Oral Maxillofac Surg. 2008 Apr;66(4):711-7