Liquid ordered and gel phases of lipid bilayers: Fluorescent probes reveal close fluidity but different hydration.

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Date publication

août 2008

Auteurs

Membres identifiés du Cancéropôle Est :
Pr MELY Yves


Tous les auteurs :
M'Baye G, Mely Y, Duportail G, Klymchenko AS

Résumé

Hydration and fluidity of lipid bilayers in different phase states were studied using fluorescent probes selectively located at the interface. The probe of hydration was a recently developed 3-hydroxyflavone derivative, which is highly sensitive to the environment, whereas the probe of fluidity was the diphenylhexatriene derivative, 1-[4-(trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene. By variation of the cholesterol content and temperature in large unilamellar vesicles composed of sphingomyelin or dipalmitoylphosphatidlycholine, we generated different phases: gel, liquid ordered (raft), liquid crystalline, and liquid disordered (considered as liquid crystalline phase with cholesterol). Forthese four phases, the hydration increases in the following order: liquid ordered

Référence

Biophys J. 2008 Aug;95(3):1217-25