Liquid ordered and gel phases of lipid bilayers: Fluorescent probes reveal close fluidity but different hydration.
Fiche publication
Date publication
août 2008
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves
Tous les auteurs :
M'Baye G, Mely Y, Duportail G, Klymchenko AS
Lien Pubmed
Résumé
Hydration and fluidity of lipid bilayers in different phase states were studied using fluorescent probes selectively located at the interface. The probe of hydration was a recently developed 3-hydroxyflavone derivative, which is highly sensitive to the environment, whereas the probe of fluidity was the diphenylhexatriene derivative, 1-[4-(trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene. By variation of the cholesterol content and temperature in large unilamellar vesicles composed of sphingomyelin or dipalmitoylphosphatidlycholine, we generated different phases: gel, liquid ordered (raft), liquid crystalline, and liquid disordered (considered as liquid crystalline phase with cholesterol). Forthese four phases, the hydration increases in the following order: liquid ordered
Référence
Biophys J. 2008 Aug;95(3):1217-25