Low cost optical detection of DNA hybridization on biochips
Fiche publication
Date publication
février 2009
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BOIREAU Wilfrid, Dr PIERALLI Christian, Dr WACOGNE Bruno
Tous les auteurs :
Pieralli C, Attoui C, Boireau W, Wacogne B
Lien Pubmed
Résumé
The detection of DNA hybridization can be either complicated or require expensive devices. Some methods use a fluorescence signal to investigate hybridization. Here, we present an optical probe based on optical fibers both for illumination and for fluorescence collection. The detection is made with a microspectrophotometer and the signal is then treated with a PC. We also developed the DNA biochips (glass plate with a gold layer functionalized with target DNA) as well as a dedicated microtank which maintains a constant flow of probe DNA over the target one. Finally, the detection of hybridization with this simple and versatile system is presented.
Référence
Laser Phys. 2009 Feb;19(2):259-64