A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.
Fiche publication
Date publication
février 2009
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MORAS Dino, Dr POTERSZMAN Arnaud
Tous les auteurs :
Abdulrahman W, Uhring M, Kolb-Cheynel I, Garnier JM, Moras D, Rochel N, Busso D, Poterszman A
Lien Pubmed
Résumé
We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs.
Référence
Anal Biochem. 2009 Feb 15;385(2):383-5