Cell Alignment Driven by Mechanically Induced Collagen Fiber Alignment in Collagen/Alginate Coatings.
Fiche publication
Date publication
février 2015
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LAVALLE Philippe, Dr VOEGEL Jean-Claude, Pr SCHAAF Pierre
Tous les auteurs :
Chaubaroux C, Perrin-Schmitt F, Senger B, Vidal L, Voegel JC, Schaaf P, Haikel Y, Boulmedais F, Lavalle P, Hemmerle J
Lien Pubmed
Résumé
Since many years, it is a major challenge to regenerate damaged tissues using synthetic or natural materials. To favor healing processes after tendon, cornea, muscle or brain injuries, aligned collagen-based architectures are of utmost interest. In this study, we define a novel aligned coating based on a collagen/alginate multilayer film. The coating exhibiting a nanofibrillar structure is cross-linked with genipin for stability in physiological conditions. By stretching collagen/alginate-coated polydimethylsiloxane substrates, we developed a versatile method to align the collagen fibrils of the polymeric coating. Assays on cell morphology and alignment were performed to investigate the properties of these films. Microscopic assessments revealed that cells align with the stretched collagen fibrils of the coating. The degree of alignment is tuned by the stretching rate (i.e. the strain) of the collagen/alginate coated elastic substrate. Such coatings are of great interest for strategies that require aligned nanofibrillar biological material as a substrate for tissue engineering.
Référence
Tissue Eng Part C Methods. 2015 Feb 6.