Evaluation of a rat knee mono-arthritis using microPET.
Fiche publication
Date publication
janvier 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GILLET Pierre, Pr OLIVIER Pierre
Tous les auteurs :
Paquet J, Maskali F, Poussier S, Scala Bertola J, Pinzano A, Grossin L, Netter P, Olivier P, Gillet P
Lien Pubmed
Résumé
AIM: Assessing the activity of synovitis, which is characterized by an increase in cell metabolism, is important for the prediction of future articular destruction in clinical and preclinical studies. To evaluate the correlation between (1)F-FDG accumulation and arthritis pathology during its establishment, we used microPET to evaluted (1)F-FDG accumulation in vivo during rat Mycobacterium wall-induced knee arthritis. METHODS: (1)F-FDG PET images of arthritic rats were acquired on days 1, 2, 3 and 7 after arthritis induction. The subjects (n=2/time) were subsequently subjected to macro-autoradiography, and (1)F-FDG accumulation was compared with histological findings. RESULTS: (1)F-FDG PET images depicted swollen joints, and (1)F-FDG accumulation increased with the progression of arthritis. Histologically, increased (1)F-FDG accumulation correlated with the pannus rather than the infiltration of inflammatory cells around the joints. CONCLUSION: (1)F-FDG accumulation in arthritis reflects proliferating pannus and inflammatory activity enhanced by inflammatory cytokines. (1)F-FDG microPET should be effective for quantifying the inflammatory activity of arthritis and/or its therapeutic response.
Référence
Biomed Mater Eng. 2010 Jan 1;20(3):195-202.