Crystallization and preliminary crystallographic analysis of eukaryotic transcription and mRNA export factor Iws1 from Encephalitozoon cuniculi.
Fiche publication
Date publication
février 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ROMIER Christophe
Tous les auteurs :
Koch M, Diebold ML, Cavarelli J, Romier C
Lien Pubmed
Résumé
Transcription elongation by eukaryotic RNA polymerase II requires the coupling of mRNA synthesis and mRNA processing and export. The essential protein Iws1 is at the interface of these processes through its interaction with histone chaperone and elongation factor Spt6 as well as with complexes involved in mRNA processing and export. Upon crystallization of the evolutionarily conserved domain of Iws1 from Encephalitozoon cuniculi, four different crystal forms were obtained. Three of the crystal forms belonged to space group P2(1) and one belonged to space group P222(1). Preliminary X-ray crystallographic analysis of one of the crystal forms allowed the collection of data to 2.5 A resolution.
Référence
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt