Fiche publication
Date publication
septembre 2025
Journal
Nanoscale advances
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky
,
Dr LEFEBVRE Olivier
,
Dr KLYMCHENKO Andrey
Tous les auteurs :
Boide-Trujillo VJ, Mittelheisser V, Liu F, Lefebvre O, Andreiuk B, Anton N, Goetz JG, Klymchenko AS
Lien Pubmed
Résumé
Lipid nanoemulsions (NEs) are promising green nanocarriers for diagnostic and therapeutic applications, but their functionalization with biomolecules, such as antibodies, remains a challenge due to liquid nature of their core. Here, we developed an original plug-and-play strategy to graft an antibody (trastuzumab) at the surface of NEs, using components generally recognized as safe (GRAS). We synthesized a reactive 4-nitrophenyl carbonate of cholesterol (NPC-Chol) and a Biotin-PEG-Lysine linker, which can react within one-pot formulation to form an amphiphilic carbamate Biotin-PEG-Cholesterol. The cholesterol ensures anchorage of the linker, which effectively exposes biotin moiety at the surface of NEs for further antibody grafting using a biotin-neutravidin coupling. The reaction between the Biotin-PEG-Lysine linker and NPC-Chol was confirmed by H-NMR and absorption spectroscopy. The obtained biotinylated 50-nm NEs loaded with a near-infrared dye were successfully targeted to neutravidin-coated glass surfaces and imaged at the single-droplet level. The biotinylated NEs bearing the trastuzumab antibody targeted specifically HER2-amplified breast cancer models HCC-1954 and SKBR3, in contrast to control MDA-MB-231 (HER2-low) cells. Altogether, our study proposes an efficient methodology for grafting antibodies to the surface of NEs, which offers new opportunities of application of these green nanocarriers in biomedicine.
Référence
Nanoscale Adv. 2025 09 23;:
