Proximity Ligation in Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy in Cancer Cells.
Fiche publication
Date publication
janvier 2022
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GUITTAUT Michaël, Dr HERVOUET Eric, Dr PEIXOTO Paul
Tous les auteurs :
Peixoto P, Guittaut M, Hervouet E
Lien Pubmed
Résumé
Proximity ligation in situ assay (PLISA) is a powerful method to quantify endogen protein-protein interactions in cells and simultaneously identify localization of these interactions. PLISA can be used to quantify autophagy flux and can as well be adapted to assess global autophagy (SQSTM1/P62-LC3B interaction) or specific autophagy, such as mitophagy (NIX-LC3B). Here, we describe a step-by-step method to monitor autophagy using PLISA in adherent cancer cells.
Mots clés
Amplification, Autophagy, Confocal microscopy, LC3B, Ligation, PLISA
Référence
Methods Mol Biol. 2022 ;2543:167-178