Receptor-mediated endocytosis of the intrinsic factor-cobalamin complex in HT 29, a human colon carcinoma cell line.
Fiche publication
Date publication
février 1992
Journal
FEBS letters
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GUEANT Jean-Louis, Pr SCHOHN Hervé
Tous les auteurs :
Guéant JL, Masson C, Schohn H, Girr M, Saunier M, Nicolas JP
Lien Pubmed
Résumé
A HT 29 cell line derived from human colonic carcinoma was shown to express the intrinsic factor receptor, with about 5000 binding sites per cell and an association constant of 20 x 10(9) 1/mol at pH 7.4 and 4 degrees C. The number of binding sites increased dramatically between 7 and 10 days of culture time. Endocytosis of the intrinsic factor-cobalamin-receptor complex was inhibited by two ways: at 4 degrees C and at 37 degrees C by incubating the cells with vinblastine, monensin and chloroquine. The plasma membrane receptor was cross-linked to [57Co]cobalamin-intrinsic factor and solubilized with Triton X-100. The cross-linked complex had a relative molecular mass of 330 kDa in native PAGE.
Mots clés
Chloroquine, pharmacology, Chromatography, Gel, Colonic Neoplasms, Electrophoresis, Polyacrylamide Gel, Endocytosis, Humans, Intrinsic Factor, metabolism, Kinetics, Receptors, Cell Surface, metabolism, Tumor Cells, Cultured, Vitamin B 12, metabolism
Référence
FEBS Lett.. 1992 Feb;297(3):229-32