Intrinsic factor receptor during fetal development of the human intestine.

Fiche publication


Date publication

août 1992

Journal

The Biochemical journal

Auteurs

Membres identifiés du Cancéropôle Est :
Pr GUEANT Jean-Louis, Pr SCHOHN Hervé


Tous les auteurs :
Schohn H, Guéant JL, Leheup B, Saunier M, Grignon G, Nicolas JP

Résumé

Intrinsic factor receptor activity was observed in mucosal homogenates from whole small intestine and colon of 10-19-week fetuses, whereas it was only detected in the distal part of the small intestine of a 25-week fetus. The receptor-intrinsic factor-cobalamin complex was eluted into the void-volume position when ileum mucosal extract was assayed for receptor activity by gel filtration after incubation with either fetal gastric extract or human gastric juice. The intrinsic-factor-binding capacity of intestinal mucosal extracts ranged from 2.6 to 30.5 fmol/mg and was correlated with the gestational age of six fetuses. The dissociation constant of the receptor for the intrinsic factor-cobalamin complex was estimated at 0.24-0.36 nM at pH 7.4. In conclusion, intrinsic-factor-receptor activity was detected in the whole intestine in 10-19-week fetuses, whereas it was only present in the distal ileum at the end of fetal development.

Mots clés

Chromatography, Gel, Colon, embryology, Embryonic and Fetal Development, physiology, Fetus, Gestational Age, Humans, Intestinal Mucosa, embryology, Intestine, Small, embryology, Intrinsic Factor, metabolism, Kinetics, Radioligand Assay, Receptors, Cell Surface, isolation & purification, Receptors, Peptide

Référence

Biochem. J.. 1992 Aug;286 ( Pt 1):153-6