How to quantify microparticles in RBCs? A validated flow cytometry method allows the detection of an increase in microparticles during storage.
Fiche publication
Date publication
mars 2017
Journal
Transfusion
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GARNACHE-OTTOU Francine, Pr SEILLES Estelle, Dr ANGELOT-DELETTRE Fanny
Tous les auteurs :
Gamonet C, Mourey G, Aupet S, Biichle S, Petitjean R, Vidal C, Pugin A, Naegelen C, Tiberghien P, Morel P, Angelot-Delettre F, Seilles E, Saas P, Bardiaux L, Garnache-Ottou F
Lien Pubmed
Résumé
The procoagulant and proinflammatory microparticles (MPs) released during storage of packed red blood cells (pRBCs) can potentially modify transfusion benefits. A robust method to quantify MPs in pRBCs is needed to evaluate their impact in clinical trials.
Mots clés
Blood Preservation, Cell-Derived Microparticles, Cryopreservation, Erythrocytes, Female, Flow Cytometry, methods, Humans, Male, Time Factors
Référence
Transfusion. 2017 Mar;57(3):504-516