Fiche publication
Date publication
septembre 2026
Journal
Applied and environmental microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CARAPITO Christine
,
Dr HEINTZ Dimitri
,
Mr ALIOUA Abdelmalek
,
Mme KOECHLER Sandrine
Tous les auteurs :
El Nayal A, Sangal V, Alioua A, Hirschler A, Koechler S, Heintz D, Carapito C, Van Dorsselaer A, Ismail W
Lien Pubmed
Résumé
Recent proteomics and metabolomics studies have confirmed the significance of understanding the fuel biodesulfurization phenotype at a global scale beyond the desulfurization 4S pathway. To reveal the transcriptomic adaptations in the model strain IGTS8 under biodesulfurization conditions, we conducted a time series comparative RNA-seq study using cultures of IGTS8 grown on either dibenzothiophene (DBT) or MgSO as the sulfur source. The RNA-seq data revealed many differentially expressed genes (DEGs) depending on the sulfur source and growth phase, and the strongest transcriptomic response was evident during the late-log phase. In total, 6,690 transcripts were detected in the DBT and MgSO cultures, representing 99.5% of the total protein-coding genes. A substantial fraction of the DEGs encodes ABC-type transporters and hypothetical proteins. In the DBT culture, 623 DEGs were upregulated, and 587 DEGs were downregulated. Generally, the DEGs covered key physiological processes, such as stress response, regulation of gene expression, uptake of nutrients, metabolism of lipids, amino acids, carbohydrates, proteins, nucleotides, cell division, and cell wall biosynthesis/degradation. While genes of carbohydrate metabolism were mostly downregulated in the DBT culture, those encoding amino acid and fatty acid degradation, as well as energy conservation, were upregulated. Moreover, genes encoding mobile genetic elements, C1 metabolism enzymes, carbonic anhydrase, and nitrile hydratase were upregulated under biodesulfurization conditions. In contrast, dihydrofolate reductase was strongly downregulated. The growth phase-dependent transcriptomic rearrangements under biodesulfurization conditions appear to prioritize cellular homeostasis and protection rather than proliferation.
Mots clés
4S pathway, dibenzothiophene, mycofactocin, stress response, sulfur metabolism, transcriptomics
Référence
Appl Environ Microbiol. 2026 09 23;:e0154926
