Analytical model enabling the correction of absorption and scattering in dual-color ratiometric multiphoton microscopy of brain tissue.

Fiche publication

Date publication

juillet 2026

Journal

Neurophotonics

Auteurs

Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul

Tous les auteurs :
Chaigneau E, Tournissac M, Walter S, Collot M, Charpak S

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/42694932

Résumé

Multiphoton microscopy with fluorescent indicators enables the monitoring of biomarker dynamics through changes in fluorescence intensity. However, quantitative interpretation remains challenging because fluorescence signals are highly sensitive to experimental conditions. Dual-color ratiometric approaches improve quantification, yet wavelength-dependent absorption and scattering of emitted photons can still introduce significant bias.