Fiche publication
Date publication
avril 2023
Journal
The CRISPR journal
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CULLOT Grégoire
Tous les auteurs :
Cullot G, Amintas S, Karembé L, Prouzet-Mauléon V, Rébillard J, Boureau L, Cappellen D, Bedel A, Moreau-Gaudry F, Dulucq S, Dabernat S, Turcq B
Lien Pubmed
Résumé
Advances in molecular medicine have placed nucleic acid detection methods at the center of an increasing number of clinical applications. Polymerase chain reaction (PCR)-based diagnostics have been widely adopted for their versatility, specificity, and sensitivity. However, recently reported clustered regularly interspaced short palindromic repeats-based methods have demonstrated equivalent to superior performance, with increased portability and reduced processing time and cost. In this study, we applied Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) technology to the detection of oncogenic rearrangements. We implemented SHERLOCK for the detection of mRNA, a hallmark of chronic myeloid leukemia (CML), and DNA oncogenic alleles, frequently detected in glioblastoma and non-small cell lung cancer (NSCLC). SHERLOCK enabled rapid, sensitive, and variant-specific detection of and alterations. Compared with the gold-standard PCR-based methods currently used in clinic, SHERLOCK achieved equivalent to greater sensitivity, suggesting it could be a new tool in CML and NSCLC, to detect low level of molecular residual disease.
Mots clés
Humans, Fusion Proteins, bcr-abl, genetics, Carcinoma, Non-Small-Cell Lung, diagnosis, Lung Neoplasms, diagnosis, CRISPR-Cas Systems, Gene Editing, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, diagnosis, ErbB Receptors, genetics
Référence
CRISPR J. 2023 04;6(2):140-151
