Integrating allele-specific PCR with CRISPR-Cas13a for sensitive KRAS mutation detection in pancreatic cancer.

Fiche publication

Date publication

octobre 2024

Journal

Journal of biological engineering

Auteurs

Membres identifiés du Cancéropôle Est :
Dr CULLOT Grégoire

Tous les auteurs :
Amintas S, Cullot G, Boubaddi M, Rébillard J, Karembe L, Turcq B, Prouzet-Mauléon V, Bedel A, Moreau-Gaudry F, Cappellen D, Dabernat S

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/39354555

Résumé

The clustered regulatory interspaced short palindromic repeats (CRISPR)-Cas13a system has strong potential for highly sensitive detection of exogenous sequences. The detection of KRAS point mutations with low allele frequencies may prove powerful for the formal diagnosis of pancreatic ductal adenocarcinoma (PDAC).