Tuning of the RBR1-E2F/DP transcriptional module by the F-box protein FBL17.

Date publication

février 2026

Journal

Science advances

Auteurs

Membres identifiés du Cancéropôle Est :
Mr HAMMANN Philippe

Tous les auteurs :
Espanet J, He X, Pan T, Gentric N, Potuschak T, Desvoyes B, Hammann P, Chicher J, Brik R, Lechner E, Latrasse D, Pettkó-Szandtner A, Gutierrez C, Raynaud C, Magyar Z, Benhamed M, Yan S, Noir S, Genschik P

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/41706863

Résumé

F-box proteins of SCF E3 ligases have been documented to control the abundance of numerous critical regulatory proteins. In , one of them, F-BOX-LIKE17 (FBL17), stands out for playing a key role in DNA replication, DNA damage, and, more recently, for the control of cell size. null mutants exhibit severe cellular defects leading to lethality. However, the molecular mechanisms by which FBL17 operate remain poorly understood. Here, we show that FBL17 interacts with different components of the RETINOBLASTOMA-RELATED1/E2F module and is involved in the protein turnover of E2Fa and E2Fb. However, mutations in or do not alleviate the severe phenotype but worsen it. By contrast, it is the accumulation of the transcriptional repressor E2Fc that causes mutant lethality. Our results highlight a key role for FBL17 in modulating the transcriptional control of E2F target genes ensuring precise control of cell cycle progression and avoiding uncontrolled DNA damage response.

Mots clés

Arabidopsis Proteins, metabolism, Arabidopsis, genetics, E2F Transcription Factors, metabolism, F-Box Proteins, metabolism, Gene Expression Regulation, Plant, Mutation, Transcription, Genetic, Protein Binding, DNA Damage

Référence

Sci Adv. 2026 02 20;12(8):eadz2439