Fiche publication
Date publication
janvier 2026
Journal
eLife
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOUMON Yannick
,
Dr SPECHT Alexandre
,
Dr GRUTTER Thomas
Tous les auteurs :
Arnould B, Martz A, Belzanne P, Peralta FA, Cevoli F, Hovhannisyan V, Goumon Y, Hosy E, Specht A, Grutter T
Lien Pubmed
Résumé
ATP-gated purinergic P2X7 receptors are crucial ion channels involved in inflammation. They sense abnormal ATP release during stress or injury and are considered promising clinical targets for therapeutic intervention. However, despite their predominant expression in immune cells such as microglia, there is limited information on P2X7 membrane expression and regulation during inflammation at the single-molecule level, necessitating new labeling approaches to visualize P2X7 in native cells. Here, we present , an unbiased, affinity-guided P2X7 chemical labeling reagent that selectively and covalently biotinylates endogenous P2X7 in BV2 cells, a murine microglial cell line, allowing subsequent labeling with streptavidin-Alexa 647 tailored for super-resolution imaging. We uncovered a nanoscale microglial P2X7 redistribution mechanism where evenly spaced individual receptors in quiescent cells undergo upregulation and clustering in response to the pro-inflammatory agent lipopolysaccharide and ATP, leading to synergistic interleukin-1β release. Our method thus offers a new approach to revealing endogenous P2X7 expression at the single-molecule level.
Mots clés
biochemistry, chemical biology, human, human HEK293T cells, mouse, mouse BV2 cells, neuroscience, rat, rat P2X sequences
Référence
Elife. 2026 01 9;14:
