Fiche publication
Date publication
novembre 2025
Journal
Nucleic acids research
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
,
Dr MARCHAND Virginie
Tous les auteurs :
Fruchard L, Sudol C, Rouard C, Treffkorn-Maurau A, Hardy L, Bos J, Duchateau M, Giai Gianetto Q, Matondo M, Bonhomme F, Thuillier Q, Marchand V, Motorin Y, Bregeon D, Mazel D, Hamdane D, Baharoglu Z
Lien Pubmed
Résumé
RNA modifications play a fundamental role in regulating essential cellular processes, including translation fidelity and stress adaptation. While these modifications are installed post-transcriptionally by specialized enzymes, their broader functional roles remain largely unexplored. Here, we uncover an unexpected function for the Vibrio cholerae tRNA dihydrouridine synthase B (VcDusB) beyond its canonical role in tRNA dihydrouridylation. We show that deletion of dusB severely compromises V. cholerae resistance to oxidative stress, not through the loss of tRNA modification, but via disruption of an intrinsic NADPH oxidase activity. Mutational analyses reveal that DusB redox function is essential for survival under oxidative stress. Proteomic and transposon insertion sequencing analysis further linked DusB to NADPH homeostasis and metabolic reprogramming during stress adaptation. These findings redefine DusB as a bifunctional enzyme coupling tRNA modification to redox regulation, expanding the functional repertoire of RNA-modifying enzymes in stress adaptation. More broadly, this work paves the way for exploring the evolutionary versatility of tRNA-modifying enzymes, suggesting that their functions extend far beyond RNA metabolism to direct integration of translational control with cellular redox state.
Mots clés
Oxidative Stress, genetics, RNA, Transfer, metabolism, Vibrio cholerae, genetics, Oxidation-Reduction, NADP, metabolism, Bacterial Proteins, metabolism, RNA Processing, Post-Transcriptional
Référence
Nucleic Acids Res. 2025 11 26;53(22):
