Fiche publication
Date publication
novembre 2025
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
,
Dr MARCHAND Virginie
Tous les auteurs :
Weber M, Raorane K, Grampp CJ, Bourguignon V, Kilz LM, Glänzer D, Marchand V, Kreutz C, Motorin Y, Helm M
Lien Pubmed
Résumé
RNA oxidation is an important yet understudied process, partly because methods to localize oxidized residues in RNA are lacking. We introduce OAbSeq, a deep-sequencing approach that maps oxidized sites with high sensitivity by exploiting aniline-induced strand scission at noncanonical nucleosides to generate unique ligation-competent fragments utilized for library preparation. Applied to yeast RNA, OAbSeq detects widespread signals predominating at purines, especially at guanosines. Exogenous oxidation increased signal intensity but preserved the guanosine-dominated pattern. Parallel quantification of 8-oxoguanosine (oxoG) and abasic sites revealed that abasic sites are more abundant than oxoG following oxidative treatment in vitro and under physiological conditions. These data support a model in which guanosine oxidation proceeds via transient oxoG yielding abasic sites that can be mapped at nucleotide resolution by OAbSeq. Our findings also suggest abasic sites may be a more informative marker of RNA oxidative damage than oxoG, facilitating studies of RNA oxidation dynamics in cells.
Mots clés
Guanosine, analogs & derivatives, Oxidation-Reduction, Saccharomyces cerevisiae, genetics, Hypochlorous Acid, chemistry, RNA, Fungal, chemistry, High-Throughput Nucleotide Sequencing, methods, RNA, chemistry
Référence
Nat Commun. 2025 11 21;16(1):10251
