Fiche publication
Date publication
mars 2015
Journal
eLife
Auteurs
Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul
Tous les auteurs :
Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonné S, Häusser M, Feltz A, Mallet JM
Lien Pubmed
Résumé
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.
Mots clés
calcium, cell biology, click chemistry, fluorescence, mouse, neuroscience, two-photon imaging
Référence
Elife. 2015 03 31;4:
