Fiche publication
Date publication
octobre 2015
Journal
Chemical science
Auteurs
Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul
Tous les auteurs :
Despras G, Zamaleeva AI, Dardevet L, Tisseyre C, Magalhaes JG, Garner C, De Waard M, Amigorena S, Feltz A, Mallet JM, Collot M
Lien Pubmed
Résumé
Monitoring intracellular pH has drawn much attention due to its undeniably important function in cells. The widespread development of fluorescent imaging techniques makes pH sensitive fluorescent dyes valuable tools, especially red-emitting dyes which help to avoid the overcrowded green end of the spectral band. Herein, we present H-Rubies, a family of pH sensors based on a phenol moiety and a X-rhodamine fluorophore that display a bright red fluorescence upon acidification with p values spanning from 4 to 9. Slight structural modifications led to dramatic changes in their physicochemical properties and a relationship between their structures, their ability to form -aggregates, and their apparent p was established. While molecular form H-Rubies can be used to monitor mitochondrial acidification of glioma cells, their functionalised forms were linked click chemistry to dextrans or microbeads containing a near infrared Cy5 (Alexa-647) in order to provide ratiometric systems that were used to measure respectively the phagosomal and endosomal pH in macrophages (RAW 264.7 cells) using flow cytometry.
Référence
Chem Sci. 2015 10 1;6(10):5928-5937
