Fiche publication
Date publication
janvier 2021
Journal
eNeuro
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GRUTTER Thomas
Tous les auteurs :
Ollivier M, Beudez J, Linck N, Grutter T, Compan V, Rassendren F
Lien Pubmed
Résumé
ATP is an extracellular signaling molecule involved in numerous physiological and pathologic processes. However, characterization of the spatiotemporal dynamic of extracellular ATP is still challenging because of the lack of sensor with appropriate specificity, sensitivity, and kinetics. Here, we report the development of biosensors based on the fusion of cation permeable ATP receptors (P2X) to genetically encoded calcium sensors [genetically encoded calcium indicator (GECI)]. By combining the features of P2X receptors with the high signal-to-noise ratio of GECIs, we generated ultrasensitive green and red fluorescent sniffers that detect nanomolar ATP concentrations and also enable the tracking of P2X receptor activity. We provide the proof of concept that these sensors can dynamically track ATP release evoked by depolarization in mouse neurons or by extracellular hypotonicity. Targeting these P2X-based biosensors to diverse cell types should advance our knowledge of extracellular ATP dynamics .
Mots clés
ATP, GECI, P2X, biosensor, purinergic signaling
Référence
eNeuro. 2021 01 28;8(1):
