Fluorescent styrenes for mitochondrial imaging and viscosity sensing.

Fiche publication

Date publication

février 2024

Journal

Photochemistry and photobiology

Auteurs

Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul

Tous les auteurs :
Singh D, Ghorpade M, Regar R, Collot M, Soppina V, Kanvah S

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/38385897

Résumé

Fluorophores bearing cationic pendants, such as the pyridinium group, tend to preferentially accumulate in mitochondria, whereas those with pentafluorophenyl groups display a distinct affinity for the endoplasmic reticulum. In this study, we designed fluorophores incorporating pyridinium and pentafluorophenyl pendants and examined their impact on sub-cellular localization. Remarkably, the fluorophores exhibited a notable propensity for the mitochondrial membrane. Furthermore, these fluorophores revealed dual functionality by facilitating the detection of viscosity changes within the sub-cellular environment and serving as heavy-atom-free photosensitizers. With easy chemical tunability, wash-free imaging, and a favorable signal-to-noise ratio, these fluorophores are valuable tools for imaging mitochondria and investigating their cellular processes.