Fiche publication
Date publication
septembre 2024
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul
Tous les auteurs :
Tournissac M, Chaigneau E, Pfister S, Aydin AK, Goulam Houssen Y, O'Herron P, Filosa J, Collot M, Joutel A, Charpak S
Lien Pubmed
Résumé
Neurovascular coupling (NVC), which mediates rapid increases in cerebral blood flow in response to neuronal activation, is commonly used to map brain activation or dysfunction. Here we tested the reemerging hypothesis that CO generated by neuronal metabolism contributes to NVC. We combined functional ultrasound and two-photon imaging in the mouse barrel cortex to specifically examine the onsets of local changes in vessel diameter, blood flow dynamics, vascular/perivascular/intracellular pH, and intracellular calcium signals along the vascular arbor in response to a short and strong CO challenge (10 s, 20%) and whisker stimulation. We report that the brief hypercapnia reversibly acidifies all cells of the arteriole wall and the periarteriolar space 3-4 s prior to the arteriole dilation. During this prolonged lag period, NVC triggered by whisker stimulation is not affected by the acidification of the entire neurovascular unit. As it also persists under condition of continuous inflow of CO, we conclude that CO is not involved in NVC.
Mots clés
Animals, Carbon Dioxide, metabolism, Neurovascular Coupling, physiology, Mice, Cerebrovascular Circulation, physiology, Hypercapnia, metabolism, Vibrissae, physiology, Male, Mice, Inbred C57BL, Hydrogen-Ion Concentration, Neurons, metabolism, Somatosensory Cortex, physiology, Arterioles, physiology
Référence
Nat Commun. 2024 09 2;15(1):7635
