Spontaneously blinking spiroamide rhodamines for live SMLM imaging of the plasma membrane.

Date publication

avril 2025

Journal

Chemical communications (Cambridge, England)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul

Tous les auteurs :
Pfister S, Walter S, Perrier A, Collot M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/40162850

Résumé

We have developed spontaneously blinking fluorescent probes based on the reversible spirolactamization of rhodamine, to efficiently image the plasma membrane (PM) of live cells with enhanced resolution using SMLM. This study demonstrates that the blinking efficiency of spiroamide PM probes is not solely governed by their p; the presence of a charged polar group on the amide should also be taken into account.

Mots clés

Rhodamines, chemistry, Cell Membrane, chemistry, Fluorescent Dyes, chemistry, Spiro Compounds, chemistry, Humans, Amides, chemistry, Microscopy, Fluorescence, Molecular Structure, Animals

Référence

Chem Commun (Camb). 2025 04 17;61(33):6170-6173