Fiche publication
Date publication
octobre 2025
Journal
Journal of cell science
Auteurs
Membres identifiés du Cancéropôle Est :
Dr COLLOT Mayeul
Tous les auteurs :
Zilliox M, Collot M, Charrier B
Lien Pubmed
Résumé
Living cells of brown algae are difficult to observe in 3D because pigments such as fucoxanthin and chlorophyll diffract light. Furthermore, at the beginning of their life, brown algae develop slowly in seawater. To gain insight into the 3D shape and size of brown algal cells during embryogenesis, we designed a fluorescence probe that labels the plasma membrane efficiently and selectively. Styryl benzoindoleninium sulfonate (SBIS) is a bright orange fluorogenic probe that is soluble and virtually non-emissive in seawater and is activated upon binding to the plasma membrane. Unlike Calcofluor White, SBIS enables observation of cells at thicknesses of up to 25 µm. More importantly, SBIS allows 3D observation of the cells in the growing uniseriate filaments of Ectocarpus sp., the polystichous filaments of Sphacelaria rigidula and the cellular monolayered lamina of Saccharina latissima over periods of up to 7 days. Altogether, these properties allow visualization of entire cell contours in living brown algae, making the study of early development at the cellular level in 4D now possible in these marine organisms.
Mots clés
Ectocarpus, Saccharina, Sphacelaria, 3D microscopy, Brown algae, Cell growth, Cell shape, Confocal microscopy, Light-sheet microscopy, Live cell imaging, Plasma membrane probes, Seaweeds
Référence
J Cell Sci. 2025 10 1;138(19):
