Fiche publication
Date publication
juillet 2025
Journal
Cell reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MOLINA Nacho
Tous les auteurs :
Nariya MK, Santiago-Algarra D, Tassy O, Cerciat M, Ye T, Riba A, Molina N
Lien Pubmed
Résumé
The cell cycle is a tightly regulated process that requires precise temporal expression of thousands of cell-cycle-dependent genes. However, the genome-wide dynamics of mRNA metabolism throughout the cell cycle remain uncharacterized. Here, we combined single-cell multiome sequencing, biophysical modeling, and deep learning to quantify rates of mRNA transcription, splicing, nuclear export, and degradation. Our approach revealed that both transcriptional and post-transcriptional processes exhibit distinct oscillatory waves at specific cell cycle phases, with post-transcriptional regulation playing a prominent role in shaping mRNA accumulation. We also observed dynamic changes in chromatin accessibility and transcription factor binding footprints, identifying key regulators underlying the oscillatory dynamics of mRNA. Taken together, the results of our approach uncovered a high-resolution map of RNA metabolism dynamics and chromatin accessibility, offering new insights into the temporal control of gene expression in proliferating cells.
Mots clés
(post-)transcriptional kinetics, CP: Molecular biology, cell cycle, chromatin accessibility dynamics, gene expression dynamics, mESCs, mRNA metabolism, single-cell multiomics, trajectory inference
Référence
Cell Rep. 2025 07 31;44(8):116089
