Differential Modulation of Endothelial Cell Functionality by LRP1 Expression in Fibroblasts and Cancer-Associated Fibroblasts via Paracrine signals and Matrix Remodeling.

Fiche publication

Date publication

mai 2025

Journal

Matrix biology : journal of the International Society for Matrix Biology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr DEDIEU Stéphane , Dr LANGLOIS Benoît , Dr SALESSE Stéphanie , Dr TERRYN Christine

Tous les auteurs :
Martin J, Falaise A, Faour S, Terryn C, Hachet C, Thiébault É, Huber L, Nizet P, Rioult D, Jaffiol R, Salesse S, Dedieu S, Langlois B

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/40379110

Résumé

LRP1 is a multifunctional endocytosis receptor involved in the regulation of cancer cell aggressiveness, fibroblast phenotype and angiogenesis. In breast cancer microenvironment, cancer-associated fibroblasts (CAFs) play a crucial role in matrix remodeling and tumor niche composition. LRP1 expression was described in fibroblasts and CAFs but remains poorly understood regarding its impact on endothelial cell behavior and angiocrine signaling. We analyzed the angio-modulatory effect of LRP1 expression in murine embryonic fibroblasts (MEFs) and breast cancer-educated CAF cells. We employed conditioned media and fibroblast-derived matrices to model fibroblastic cells angiogenic effects on human umbilical vein endothelial cells (HUVEC). Neither the extracellular matrix assembled by MEFs knock-out for LRP1 (PEA-13) nor their secretome modify the migration of HUVEC as compared to wild-type. Conversely, LRP1-deficient CAF secretome and matrices stimulate endothelial cell migration. Using spheroids, we demonstrate that PEA-13 secretome does not affect HUVEC angio-invasion. By contrast, CAF secretome invalidated for LRP1 stimulates endothelial sprouting as compared to controls. In addition, it specifically stabilized peripheral VE-cadherin-mediated endothelial cell junctions. A global proteomic analysis revealed that LRP1 expression in CAFs orchestrates a specific mobilization of secreted matricial components, surface receptors and membrane-associated proteins at the endothelial cell surface, thereby illustrating the deep influence exerted by LRP1 in angiogenic signals emitted by activated fibroblasts.