Fiche publication
Date publication
avril 2025
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
,
Dr MARCHAND Virginie
Tous les auteurs :
Leroy E, Challal D, Pelletier S, Goncalves C, Menant A, Marchand V, Jaszczyszyn Y, van Dijk E, Naquin D, Andreani J, Motorin Y, Palancade B, Rougemaille M
Lien Pubmed
Résumé
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA mamRNA and carrying two independent cellular functions. On the one hand, snR107 guides site-specific 25S rRNA 2'-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2'-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.
Mots clés
RNA, Small Nucleolar, metabolism, RNA, Ribosomal, metabolism, Methylation, Schizosaccharomyces, genetics, Schizosaccharomyces pombe Proteins, metabolism, Gametogenesis, genetics, RNA Processing, Post-Transcriptional, Gene Expression Regulation, Fungal, RNA Precursors, metabolism, RNA, Fungal, metabolism, RNA-Binding Proteins, metabolism, RNA, Long Noncoding, genetics, mRNA Cleavage and Polyadenylation Factors
Référence
Nat Commun. 2025 04 5;16(1):3250
