Fiche publication
Date publication
mars 2025
Journal
NAR cancer
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri
,
Dr MARCHAND Virginie
Tous les auteurs :
Morin C, Paraqindes H, Van Long FN, Isaac C, Thomas E, Pedri D, Pulido-Vicuna CA, Morel AP, Marchand V, Motorin Y, Carrere M, Auclair J, Attignon V, Pommier RM, Ruiz E, Bourdelais F, Catez F, Durand S, Ferrari A, Viari A, Marine JC, Puisieux A, Diaz JJ, Moyret-Lalle C, Marcel V
Lien Pubmed
Résumé
The epithelial-mesenchymal transition (EMT) is a dynamic transdifferentiation of epithelial cells into mesenchymal cells. EMT programs exhibit great diversity, based primarily on the distinct impact of molecular activities of the EMT transcription factors. Using a panel of cancer cell lines and a series of 71 triple-negative primary breast tumors, we report that the EMT transcription factor ZEB1 modulates site-specific chemical modifications of ribosomal RNA (rRNA). Overexpression of ZEB1 and ZEB2, but not TWIST1, decreased the level of 2'--ribose methylation (2'Ome) of 28S rRNA at position Um2402. ZEB1 overexpression specifically reduced the expression of the corresponding C/D box small nucleolar RNAs (snoRNAs) SNORD143/144, which guide the rRNA 2'Ome complex at the 28S_Um2402 site. During ZEB1-induced EMT induction/reversion, the levels of both 2'Ome at 28S_Um2402 and SNORD143/144 were dynamically comodulated. Taken together, these data demonstrate that 2'Ome rRNA epitranscriptomics is a novel marker of ZEB1-induced EMT.
Mots clés
Zinc Finger E-box-Binding Homeobox 1, genetics, Epithelial-Mesenchymal Transition, genetics, Humans, RNA, Ribosomal, 28S, genetics, Female, Methylation, Cell Line, Tumor, Breast Neoplasms, genetics, Epigenesis, Genetic, RNA, Small Nucleolar, genetics, Ribose, metabolism, Zinc Finger E-box Binding Homeobox 2, genetics, Gene Expression Regulation, Neoplastic, Transcriptome
Référence
NAR Cancer. 2025 03;7(1):zcaf001
